Hello, I am trying to preform a screen for TRPV1 activation. I am using HEK293 constantly expressing TRPV1. bought the Fluo-4 kit from abcam, using their protocol that includs 1h incubation in 37o. after 1h and even 0.5h i get high fluorescent read, then when i add the treatments there is a significant decreas. when I did the first experiments everything was allright. any suggestions? what am I doing wrong?