Maayan Freiberg Dadon

2 Questions 2 Answers 0 Followers

Questions related from Maayan Freiberg Dadon

Using Fluo-4 to detect TRPV1 activation- why do i get high fluorescent read befor I'm adding the treatments?

Hello, I am trying to preform a screen for TRPV1 activation. I am using HEK293 constantly expressing TRPV1. bought the Fluo-4 kit from abcam, using their protocol that includs 1h incubation in...

31 March 2022 6,599 0 View

I was preforming the acetylcholinesterase in vitro inhibition screening and the assay didnt work. anny suggesstions?

Hello everyone, I was preforming ths assay using Ellman's method and nothing happend. I'v added Acetylcholine iodide to initiate the reaction ad prefoemd it at RT. any recommendations what I did...

20 June 2021 3,221 5 View