01 January 2020 2 9K Report

Hi All,

I was wondering if anyone had used the chormotek gfp trap magenetic agarose bead kit for purification of GFP tagged nuclear proteins.

I am attempting to purify a gfp-tagged protein (~230kD) from transiently transfected HEK293T cells (transfection efficiency is high and the cells are blazing). I have had some issues with large clumps of unlysed cells/DNA after adding RIPA buffer (add more DNase or too many cells?).

Has anyone performed this protocol for nuclear proteins and have any additions to the chromotek protocol which were helpful?

Thanks,

Aidan

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