I actually have two queries. Biochemical studies suggested presence of an enzyme in an organism but the gene encoding the enzyme is not known. I would like to find out gene candidates based on homology search/sequence similarity, using sequences of similar enzymes present in other organisms. My questions are:

1. What points should I consider to select an already known gene/protein for use in homology search

2. To find out the orthologue of the known gene/prtoein by bioinformatics, which database/software should I use?

Please suggest papers/websites/softwares for beginners.

Thanks!

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