you cannot use plastic cuvettes in UV region. You will experience too much fluctuation of absorbance. Rather used quartz cuvettes, because are stable in that range.

According to the answers of Harald and Thandazile, please note that solvents and cuvette materials used in UV-Vis have a CUTOFF.

P.E Acetone has an UV-absorbance cutoff at 329 nm, DMF 267 nm and EtOH 205 nm water (pure) 190 nm.

Materials have a cutoff too:

Glass 380-780 nm

Plastic 380-780 nm

Methacrylate 250-1100 nm

Quartz (fused) 200-2500nm

If you use microtiter plate:

please read the added file

and

please read the following answer of a question posted in research gate 3 years ago.

Jay Martiniuk

https://www.researchgate.net/post/Which_polystyrene_microplate_should_

I_use_for_UV_absorbance_340_nm

JRG

Thanks a lot Dr. Harald Kühnel, Dr. Thandazile Abram Mzini, and Dr. Jean Rene Grezes, for the suggestions!

I was thinking, if there was any change, to use the glass cuvettes, quartz cuvettes are comparatively much expensive and I need a few!

Plastic cuvettes are functional only at 380-780 wavelength 😁