What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Sample is in a powdered form - how do I prepare for DLS?

I have this question from @Anurag Nath posted on an existing thread: https://www.researchgate.net/post/What_are_some_effective_sample_preparation_techniques_for_DLS_study To avoid confusing the...

06 January 2021 4,856 2 View

Does anyone have an insight into the behavioural response of fishes to moving wind turbine blades as seen through the air-water interface?

I am interested to find out if wind turbines located close to rivers or lakes, or perhaps even in marine locations, are a potential problem for fish. This seems to be a very under-researched area...

04 January 2021 3,468 2 View

Why is my TiO2 sol milky?

@Krunal Dholiya has sent me this question which I post below so he can get the benefit of many experts. Dear, I am preparing a thin film using TiO2 sol but i m fasing problem, with time it is...

23 November 2020 1,188 2 View

Zeta potential and concentration?

I have a question from @Pratima Adhikari that I will post on the open forum so all experts can contribute. Hi Alan, I hope you are fine and healthy. I have a question for you. I am currently...

22 October 2020 3,377 3 View

Which software can be used to prepare nexus file for phylogenetics analysis in Mr Bayes?

which software can be used to prepare nexus file for phylogenetics analysis in Mr Bayes? which format is best interleaved or sequential formats? Can you please explain?

22 February 2020 4,878 3 View

Possibility of inculcating INTELLIGENT MANAGEMENT SYSTEM to Higher education system?

The impact of IMS(INTELLIGENT MANAGEMENT SYSTEM) in the industry as well as economy in multiple countries at multilevels had been creating immense impact in the way the whole system was developed...

03 December 2019 4,284 3 View

What can be the possibility of inculcating behavioral finance to the existing studies conducted?

What are the possibilities of incorporating BEHAVIOURAL FINANCE to the existing stream of studies that already gave a clear base to the subject for Phd? What are the possibilities of looking at...

03 December 2019 5,884 11 View

What should be the basic text/document to refer upon for researches(especially social sciences)?

The fact that better knowledge on basic research comes out of understanding the existing techniques and methods is the key for a fruitful research. 1.Which should be considered as the primary...

23 November 2019 3,725 3 View

What causes the stability or instability of powders in suspension?

@Talat Zakeri has asked me this question by PM. In order that she can benefit from input from many experts I post it below: Hi, I have two powders from Sasol company. one of them is alumina and...

21 April 2019 8,410 7 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

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17 February 2021 4,217 3 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View