Hi everybody,
I performed a Real-Time PCR (using SYBR green for primers) on neonatal cardiomyocytes cDNA, to evaluate the expression of MPC1 and MPC2 genes (with Actin as an internal control). For some samples, I obtained an unusual amplification curve (the ones on the left in the picture).
Does anyone know why this happened?
Thank you very much in advance,
Giulia