Until what percentage does acetonitrile remain not toxic to cells?

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Tomas Koltai

The following publication may help you t find an answer:

https://www.novapublishers.com/catalog/product_info.php?products_id=34772&osCsid=b

https://www.proimmune.com/ecommerce/pdf_files/userguide.pdf

A concentration of acetonitrile of 0,3% (V/V) should be safe according to publications.

Adam B Shapiro

My advice would be to use DMSO. The high volatility of acetonitrile makes it difficult to accurately deliver a specific volume.