I have Humulin R which is 100 units/mL, which is normally used in animals or humans. I want to use this to conduct an in vitro study in which I want to use this Humulin R to stimulate and activate insulin receptor in cells. Given some papers use nM of insulin instead of unit/mL, can anyone tell me how I can convert units/mL to nM?
Hi Yinan,
I´ve used humulin R in some experiments, and we calculated based on the information that 25U corresponded to 1 mg of insulin. This information may be usefull to aplly conversion formulaes to mg to nM. I think the worse is find out how much insulin is 1U. Hope it´s usefull. Attached a copy of the paper.
it is good to use ''graphpad prism converter''. It helped me alot to reduce my time and brain consumption in calculations.
Check this link
http://www.endmemo.com/sconvert/nmol_mlnm.php
regards
1 unit = (1/24,000)/5,808 = 7.174 nmol or 1 μU/ml = 7.174 pmol/l. The author clarified that the new World Health Organization insulin standard from 1987 has a potency of 26,000 units/g, and by taking additional factors into account the correct conversion factor is 1 μU/ml = 6.00 pmol/l.