12 Questions 10 Answers 0 Followers
Questions related from Yinan Yuan
I am looking for an anti-mouse VEGF-C / -D antibody (ideally monoclonal) that works well by IHC on paraffin-embedded mouse tissue sections. As I started my searching I came to realise that the...
14 February 2022 6,807 0 View
Hi all, This may be a really dumb question but I just want to clarify one thing when you scan your membrane for western blotting. I have a primary antibody raised in mouse, so I used Alexa Fluor...
05 July 2019 7,336 4 View
Hi all, I have been trying to measure a concentration of my exosomes from adipose-derived stem cells. I used 700 ml of conditioned media to isolate exosomes however, when I did BCA assay it gives...
01 July 2019 4,559 8 View
Hi all, I need your guys' advice as to how to increase the concentration of exosomes isolated from conditioned media. I am trying to isolate exosomes from adipose-derives stem cell. I usually...
30 May 2019 3,928 3 View
I am trying to study an effect of a small molecular inhibitor for IGF1R/IR in vitro. I want to know whether anyone has successfully detected IR and phospho-IR by IP/WB using IGF-1 as a ligand, but...
16 April 2018 5,904 2 View
I have Humulin R which is 100 units/mL, which is normally used in animals or humans. I want to use this to conduct an in vitro study in which I want to use this Humulin R to stimulate and activate...
16 April 2018 3,762 5 View
Hi all I have Actrapid Penfill human insulin solution with stock concentration of 100 IU/ml. I want to do I.P injection to a mouse at a dose of 0.75 IU/kg. However, if each mouse weighs 20 grams,...
24 October 2017 4,418 1 View
Hi all. I am planning to give mouse an I.P injection of insulin at a dose of 0.75 IU/kg, but if a mouse weighs, for example, 20 grams, the dose would be 0.015 IU per mouse. How can you possibly...
22 October 2017 8,845 4 View
Is there such a case that certain antibodies have limited access to the intra-cellular domain of its receptor and this results in a negative staining? I have been struggling trying to detect...
07 March 2017 2,837 4 View
Hi all I am having a trouble with my immunohistochemistry. I am trying to detect a secreted protein in FFPE mouse tissue sections, and I use concentration-matched goat IgG as my negative control....
01 March 2017 3,313 5 View
Hi all I used NP40 lysis buffer for HeLa cells and HEK293T cells and used the whole cell lysates to do Western blot. The main purpose was to determine what cell line express endogenous IGF-1R...
06 September 2016 2,484 4 View
Hi all, I am a final year Ph.D candidate and next week I am going to have my first postdoc interview. I would like to get some advice from experienced postdocs as to how I should prepare my...
01 January 1970 7,265 36 View
