I need to inject postnatal mice with a drug that is only soluble in DMSO, can anyone offer advice on this?

I have a drug which is only soluble in DMSO and I need to inject postnatal mice via IP for 5 days. The stock concentration of the drug is 5 mg/mL, and the needed injection dose for the mice is 70...

09 August 2020 3,370 3 View

Which standard deviation should I use when calculating standard error?

I am currently running a meta-analysis exploring effects of mindfulness interventions on creativity. I am using JASP software to run my meta-regression, which needs effect size and standard error....

09 August 2020 9,161 3 View

Can I get recommendations on articles re:: challenges of students with disabilities in higher education?

Hello all, I am specifically seeking scholarship on the history of students with disabilities, as it pertains to challenges of the past and current obstacles, to higher education inclusion,...

21 July 2020 7,964 3 View

K10/K6 and PHQ9 comparison?

Hi Is anyone aware of a publication or article discussing the convergent validity or crosswalk of the K10 and PHQ9 for a measure of depression? Many thanks

03 March 2020 8,601 1 View

Would someone be able to provide assistance with alizarin red s/mineralization assay with SaOS-2 cells?

I'm currently trying to induce and identify mineralization in my SaOS-2 cells. The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the...

26 January 2020 6,289 5 View

How to do a hydrodynamic tail vein injection of naked DNA without killing the mouse?

I am injecting a plasmid into FVB mice using the hydrodynamic tail vein protocol laid out in many papers including this one on the Jove...

06 July 2018 6,076 2 View

Help with high background staining with immunofluorescence of tumors?

I am relatively new to immunofluorescence, and I am having trouble getting my assay working. I have a murine tumor that was formalin-fixed overnight and paraffin embedded. The tumor was sectioned...

12 October 2016 1,635 6 View

How do I remove muscle from mouse rib cages?

I would like to have just the whole costal cartilage for my work. Most of the article I have found involves hand dissection and washing in phosphate buffered saline or using trypsin. Does anyone...

12 August 2016 5,938 3 View

Which statistical method should I use?

I want to do an experiment on comparing bathroom door handles of women and men to see if men are dirtier than women and if they tend to wash their hands less. I would just swab different door...

24 February 2015 1,365 8 View

Is there a protocol to deduce the specific activity of LacZ in a 10 ml culture sample of Escherichia coli?

08 February 2015 8,719 6 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View