I need to find a software package that can analyse the droplet number, size distribution, mean size, circularity etc. for this image. ImageJ is unable to process this image due to the reflective nature of the droplets which means that the droplet boundaries are not clear. Does anybody know of another image processing software that could do it?
The problem is transmitted light images (brightfield, DIC, phase contrast) do not have very clear contrast difference between object and background. This makes it difficult to do automated object detection and measurement. Could you add a fluorescent dye to the liquid and then image droplets with a fluorescent microscope? If you can do that, then getting the measurements will be easy.
Using a neural network package e.g. in python it must be a simple task.
Most probably you can find some out of the box solutions that can be trained easily for this case.
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