My question is that how can we select the unique peptides ?

Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...

26 January 2021 7,488 3 View

I am planning to start a prevalence study on CSOM in gilgit baltistan region . how shoud i calcuate the sample size ??

i need to find out the sample size as no research has been done on this topic so no stats are available and how can i tailor the research for use in hospital setting ???

19 January 2021 5,572 5 View

How to design reverse primer with ambiguous nucleotides?

How to design, reverse primer with ambiguous nucleotides?

03 January 2021 6,762 4 View

What is the outcome of research for researchers?

For the past few years I have been in doubt and a question about the nature of research always occupies a part of my mind. As researchers, we have been asking and researching for years. We may...

17 November 2020 4,505 11 View

How i can get rid of phenolic contamination (low 260/230 ratio) in RNA extraction from blood by using Gene All TRIZOL (Ribo LS)??

in this work i use the standard protocol in extraction& good sampling and use chloroform and twice wash with ethanol 75% but i have phenol contamination (low 260/230 ratio) for example: OD:...

21 September 2020 8,408 3 View

How to open supplementary data in Science Direct?

20 September 2020 8,995 2 View

How can I find some material (books, articles and etc.) to do and write a systematic literature review(SLR)?

I need to review theories of moral decision making in a systematic method to find different definitions and models of that. I have read some guidelines about the systematic review and...

30 June 2020 3,087 13 View

Relationship between STEP AP209 and CGNS?

Could you please share your knowledge/experience about the relationship between STEP AP209 (ISO 10303: AP209) and CGNS: - Does AP209 covers/supports CGNS 100%? - Where can I read more about the...

22 March 2020 6,625 4 View

After mRNA to cDNA conversion and then PCR the appearance of PCR bands on non specific position/ near to target region? What is the reason?

To find out the reason why the nonspecific PCR bands appear, either it is due to the annealing of one of the primers?

03 March 2020 2,267 4 View

Is there any labeled public dataset for COVID-19 ?

Hi, do you know any resource for accessing data-sets about the symptoms and conditions of people who tested positive or negative for the Corona virus ?

03 March 2020 508 6 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

How to do Matrix inversion using FPGA ?

I need to implement matrix inversion in hardware. The size of the matrix can be anywhere from 50 *50 to 8*8. Are ready-made standard codes available or inbuilt modules available with FPGA...

15 February 2021 4,006 3 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

How to deal with multidimensional predictor in multiple regression?

I am investigating the influence of Trait Emotional Intelligence(TEI) and Communication styles (specifically Precision and Expressiveness styles) on Academic performance. I am now in a fix as to...

10 February 2021 7,630 3 View

Schrodinger Desmond runs fine on one GPU, but crashes on other GPU?

Hi, We are having issues using Desmond on our workstation. Simulations run fine on one of our gpus ("Gpu0") but crash consistently on the other gpu ("gpu1" - which is also set up as the display...

01 February 2021 5,866 3 View

GROMACS: how do I know how many box images a particular atom has moved from from t=0 to t=N?

In GROMACS how do I know how many box images a particular atom has moved from from t=0 to t=N? LAMMPS, for example, has the option to include image flags in the dump file for x, y, and z. Is there...

30 January 2021 8,929 3 View

Why can I not enter the title of the journal?

When I upload articles the details page does not allow me to enter the names of lesser known journals. It says "type to search" but because the journal is not on the list, I am left with a blank...

26 January 2021 2,988 3 View

Antibody storage containers?

Does anyone have suggestions for opaque antibody storage boxes (ideally not cardboard) that can hold variable sizes of antibodies. We are getting by with foil wrapped tackle boxes but I was hoping...

25 January 2021 2,591 4 View

AMBER tleap solvatebox always undervalues the density, why?

I've been searching for an answer to this question for years, and I'm shocked I haven't found any discussion on any forums. Here is an example to provide more details: I am preparing a 450...

20 January 2021 7,561 2 View