Hello everyone,
I need to sterilize tween 20 to add in culture medium. which kind of syringe filter (0.22) is suitable? Can I use Cellulose acetate syringe filter?
Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...
02 August 2024 6,980 4 View
it's my first time using NVivo software.
01 August 2024 9,304 5 View
I have collect a LSV data by taking SCE as the reference electrode. But I want to convert this one to Potential with respect to Reversible Hydrogen Electrode. Please suggest me the required...
13 July 2024 7,841 2 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
Is it a source for moisture factor in different plants? I need to find moisture factor (oven dry weight / wet weight) in plants in different studies.
10 June 2024 842 0 View
the autoclave has been smelly and seem likely yellow after my synthesis and this changes may effected on other synthesis, so I want to clean PTFE from all sulfur contamination that remain on...
31 May 2024 2,985 0 View
I have a lipopeptide solution that I intend to lyophilize in a few days. Should I put it in the -20 freezer or -80? Which temperature is best for maintaining its properties?
20 May 2024 3,893 0 View
Dear researchers, I'm currently conducting a direct cyclic fatigue simulation in Abaqus, but I'm encountering difficulties in analyzing the results. Despite the simulation running, I'm struggling...
04 May 2024 6,957 2 View
Hello. My polyacrylamide gel becomes cloudy after de staining with a solution consist of (50% miliQ water + 40% methanol + 10% acetic acid) and the background of my gel does not become completely...
02 May 2024 895 2 View
I seeded 30000 ReN progenitor cell on XF96 plate which coated with matrigel. I differentiate them for 2 weeks and read OCR with inhibitors. However nothing worked. FCCP and oligomycin were same...
17 April 2024 9,012 0 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
isolation of microplastic from sludge sample using centrifugation ..
23 July 2024 6,418 0 View
I am working on implementing a Kalman filter integrated with ARMA parameters, as described in the article "Predicting Time Series Using an Automatic New Algorithm of the Kalman Filter"...
12 July 2024 8,116 2 View
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11 July 2024 8,969 1 View
Hello everyone! I would appreciate some advice regarding the filtering of structural variants. The variant caller used for discovery-“DELLY or DELLY2”using whole-genome sequencing and Whole...
09 July 2024 498 1 View
Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...
08 July 2024 2,807 0 View
I use X-ray diffraction (XRD) techniques to quantify the degree of crystallinity in my samples, referring to the following formula (CrI=Acrystal-Aamph/Atotal ). using deconvolution area methods (...
06 July 2024 6,234 9 View
Hello, I am looking for (estimated) degradation rates of cellulose in a classic anaerobic digestion setup (meso or thermophilic, WWTP sludge, 30+ days of operation) to have an idea of the % of...
01 July 2024 5,867 5 View