TURBO DNase for RNA: Heat inactivation vs bead-mediated removal?

25 November 2019 1 6K Report

For TURBO DNase, is buying the full kit that uses bead-mediated removal of the DNase worth it? Or can I just buy the TURBO DNase itself and use heat inactivation?

I'm mainly interested in using the RNA for qPCR, and maybe RNA-seq.

Craig Silver

From Thermo's website (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4393900B.pdf)

EDTA to a final concentration of 15mM will quench DNASE I, no need to remove with their kit, otherwise a phenol:chloroform extraction works too.

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