Trustworthy 18S primer?

More Nicholas Samuel Brown's questions See All

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Women often complain that their husbands never touch them unless they want sex. (Michele Weiner-Davis)

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How to use energy flexibility in inventory modeling?

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Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

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RNA Extraction Using Hot Borate Method No Longer Working?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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Khan Mohammad Imran

18S primers: forward 5′-GATGTAGTTGCTTGGGACCCA-3′ and reverse 5′-TGGAGATAACACTCTAAGCATAACTAAAGGT-3′ (size 140 bp)

Good Luck

Imran

Jan Stenlid

I am not sure that you can regard ribosomal genes as background. A more active cell would need more ribosomes, so your comparison would be against the change in activity of the whole cell. Which I guess you may be interested in.

Fabio Alexis Lefebvre

I would agree that 18S might not be the cleanest house keeping control.

I use the GETprime database of validated, exon-specific qPCR primers. Check out the link.

http://bbcftools.epfl.ch/getprime