I am seeing large ‘blotchy’ regions of DAB staining on my sections (both experimental with primary and secondary) – some as ‘rings’ around the annulus fibrosus others as patchy areas (see image). These also appear in the ‘secondary only’ negative controls.

I am trying to figure out how to troubleshoot it, but not sure where to start. If you have any insights, I would be very appreciative.

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