In my lab we want to design an indirect ELISA to detect AAV2 in mouse serum. The ELISA has been designed to run samples and standards in duplicates at 450 nm. The plate includes blanks, positive, and negative controls.

For this ELISA, we decided to test serum at two dilutions (1:200 and 1:800). The OD readings came back similar for both dilutions. Wondering if anyone had thoughts on why this would have happened. Any troubleshooting advice, would be greatly appreciated.

Thank you!