Hello all!
I have som trouble staining beta galactose in my MC3T3 cells.
Here is my protocol:
- Cells stressed with H202 treatment.
- washed twice in PBS
- fixated in 4% paraformaldehyde 5min at 4 degrees.
- washed 3times ×3min in PBS
- staining solution added (freshly made. Containing 1mg/ml X-gal, 150mM NaCl, 40mM sodium phosphate and Citric acid, 5mM potassium ferrocyanide and potassium ferricyanide, 2mM MgCl2, pH 6)
-incubate over night, 37degrees in a non CO2 chamber
What is missing or could be optimised for the staining to work?
Grateful for any help!