We try to select the best concentrations and time of exposure to generate a positive control (inflammation) in HEPG2 cells. We see differents papers and some work with 1ug/ml - 5ug/ml -10ug/ml but in really different times (4 hours -24 hours).
Some have idea to the ideal concentration and time to generate this response in this cells?
Mark Ian Fowler
I've used 1ug/ml of LPS for 24hrs and picked up a moderate increase in IL-8 by ELISA. I suppose the earlier timepoints are more suitable for PCR.
1 votes
0 thanks
Franco Campos
Mark Ian Fowler thank you very much! want to analyze by western blot different inflammatory cytokines
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA
Similar questions and discussions