Recognition of Molecules from a file containing molecular dynamics trajectory?

Can anyone recommend a good code/software/implementation to identify the change of molecules (bond creation/break, number of molecules at a given timestep, etc.)? I am currently working with XYZ...

12 January 2021 1,655 4 View

How to Generate Fluid Volumes For Axial Turbine Blades - For CFD Analysis?

I have created stator blades and rotor blades by using coordinates and used SolidWorks. However, I tried to create fluid volumes for those blades, to simulate in CFX and have not achieved yet. I...

23 October 2019 9,393 2 View

Can a single risk score be developed to predict different outcomes collectively considered as critical outcomes?

01 January 1970 154 4 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

Hi everyone! I use HaCaT cells and i transfect them with siRNA. After 48 h I extract proteins with Laemmli and RNA with trizol. I proceed with western blot to check proteins levels (and they are...

26 January 2021 5,738 3 View

How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

What Lysis for frozen tissus for nuclic acid extraction (Maxwell plus RSC FFPE kit) ?

Hello, I have a technical question for you. For RNA and DNA extraction, we use Quiagen kit with Trizol/thiocyanate de guanidine lysis. However, it's a bit long and we wanted to improve our...

14 December 2020 7,596 1 View

Most recent, reliable, optimized methods to extract both RNA and protein from flash-frozen brain tissue?

I have flash-frozen brains and am looking to extract RNA for RNA-seq, and protein for western blotting. I've seen other questions related to this...

10 December 2020 6,294 0 View

How to improve DNA extraction using TRIzol and chloroform?

02 December 2020 1,919 1 View

Trizol samples wont freeze. Can it cause problems?

Hi, everyone. I recently lysated cell culture with Trizol reagent, and I put my samples in -20C. But, some of my samples wont freeze, and I can see two separete phase appearing in them. I'm awaer,...

06 November 2020 10,002 1 View

I am trying to isolate the RNA by trizole method, the nanodrop gives the A260/230 ration above 2.0, however no bands appear when I run on gel in TBE?

Any suggestion.

20 October 2020 8,143 4 View

What is the better method for RNA/protein extraction: Trizol or Methanol/Chloroform?

We want to do transcriptome and phosphoproteome analyses of the same sample and have limited material. So a simultaneous extraction method for both (RNA and protein), such as Trizol or...

18 October 2020 1,708 1 View