Hi all,

Optimizing a T-reg suppression assay here and have a question:

Can you substitute whole PMBCs for isolated T-effectors? If so how do you determine the ratios (i.e. 1:1, 1:2) since these would not be pure T-effector/responders?

We normally do this with purified T-effector/responders, however we want to simplify our protocol for higher throughput without sacrificing too much sensitivity.

Thank you!