Is it possible to salvage smFISH following the hybridization step if done incorrectly. My protocol calls for incubation of DNA probes on top of cells overnight following fixation (4% PFA) and permeabilization (TX-100 0.5%). This is done at ideal conditions of 37C, however I left my plates in 4C. Has anyone salvaged a similar experiment? Would leaving plates at 37C now still allow for proper binding, or is this no longer possible? Plates are left in a hybridization and formamide solution. (This is the day after leaving overnight).