16 Questions 28 Answers 0 Followers
Questions related from Francesco Boldrin
Hi All, I found in the same muscular tissue lipid droplets with different appearance (different gray scale color). I would really appreciate if someone could give me some explanation about...
last month 8,122 2 View
Dear all, I am looking for a good embedding technique for Epon embedding of adypose tissue. Until now, with the procedure that we routinely use, we do not have good results, in particular...
24 October 2024 2,116 2 View
Dear all, I found in cell cultures some structures that I cannot identify I will really appreciate is someone would give me some suggestions Many thanks Francesco
10 May 2024 2,130 11 View
Dear all, I would like to know if are available the drivers of Veleta CCD camera for Win7 or higher. Many thanks Best regards Francesco Boldrin
01 December 2023 1,512 0 View
Dear all, I would ask if there are any advantages/way to use a freeze substitution method to embed samples for transmission electron microscopy in just chemical fixed samples, instead of using it...
17 November 2023 8,900 5 View
Dear all, I'm experiencing the presence of tiny spots on transmission electron microscopy pictures of muscles. Attached you will see 3 pictures of heart tissue in which all the structures (fibers,...
18 September 2023 2,595 3 View
Hi guys, I did an Epon embedding of mouse liver for trasmission electron microscopy, in order to see mitochondria cristae, but the images appear blurry and I cannot see the cristae properly (see...
22 August 2023 4,285 2 View
Dear all, I will really appreciate if someone could give me some suggestions to how to embed (fixation, postfixation, which resin) and in particular how to cut Diatoms algae, in order to...
17 January 2023 8,063 4 View
Hi guys, I’m experiencing some very small black spots in my electron micrographs. So far I noticed them in particular in tissues/cells that contain glycogen granules…at least this is what seems...
14 November 2022 336 7 View
Hi Guys, I’m having a terrible time trying to perform immunogold pre-embedding transmission electron microscopy, on monolayer cells to do CLEM. Basically, I follow the protocol suggested in many...
08 September 2020 5,760 2 View
Dear all, I'm looking for some text/atlas/papers/web sites, which show and explaing the endocytic pathways at the transmission electron microscopy level (lysosomes, heterolysosomes, phagosomes,...
29 October 2019 5,442 0 View
Hi guys, I am planning to buy a new diamond knife for routine TEM sectioning. Right now, I am using a Drukker knife and I like it very much, unfortunately I cannot find them anymore. I will really...
26 March 2019 8,232 4 View
Hi guys, I would like to do preembedding immunogold on cell cultures. I followed different protocols and I tried to localize different proteins, with both Nanogold (1.4nm) and Aurion (0.8nm) gold...
25 July 2017 5,899 9 View
Dear all, A lab asked to our electron microscopy facility to analyze whether antidepressants change autophagy in mouse hippocampus neurons with transmission electron microscopy. In order to do...
03 April 2017 1,752 6 View
Hi, I will appreciate if someone could give me a detailed protocol for fixation adn embedding adipose tissue for transmission electron microscopy, in order to see mitochondria cristae. Thank you...
07 September 2016 8,641 2 View
Hi all, I would like to batch convert simultaneously pictures taken with TIA in a FEI Tecnai electron microscope from ".emi" to ".TIFF" format, instead of doing it one by one. I will appreciate if...
27 November 2015 3,961 5 View
