8 Questions 17 Answers 0 Followers
Questions related from Francesco Boldrin
Dear all, I will really appreciate if someone could give me some suggestions to how to embed (fixation, postfixation, which resin) and in particular how to cut Diatoms algae, in order to...
17 January 2023 7,828 4 View
Hi guys, I’m experiencing some very small black spots in my electron micrographs. So far I noticed them in particular in tissues/cells that contain glycogen granules…at least this is what seems...
14 November 2022 244 7 View
Hi Guys, I’m having a terrible time trying to perform immunogold pre-embedding transmission electron microscopy, on monolayer cells to do CLEM. Basically, I follow the protocol suggested in many...
08 September 2020 5,501 2 View
Hi guys, I am planning to buy a new diamond knife for routine TEM sectioning. Right now, I am using a Drukker knife and I like it very much, unfortunately I cannot find them anymore. I will really...
26 March 2019 8,134 4 View
Hi guys, I would like to do preembedding immunogold on cell cultures. I followed different protocols and I tried to localize different proteins, with both Nanogold (1.4nm) and Aurion (0.8nm) gold...
25 July 2017 5,823 9 View
Dear all, A lab asked to our electron microscopy facility to analyze whether antidepressants change autophagy in mouse hippocampus neurons with transmission electron microscopy. In order to do...
03 April 2017 1,652 6 View
Hi, I will appreciate if someone could give me a detailed protocol for fixation adn embedding adipose tissue for transmission electron microscopy, in order to see mitochondria cristae. Thank you...
07 September 2016 8,555 2 View
Hi all, I would like to batch convert simultaneously pictures taken with TIA in a FEI Tecnai electron microscope from ".emi" to ".TIFF" format, instead of doing it one by one. I will appreciate if...
27 November 2015 3,875 5 View
