Hello! I am looking for a transgenic mouse line that expresses constitutively a red fluorescent marker (mruby or tdTomato) targeting cellular nuclei that can be observed in vivo with two-photon microscopy. I look for something like this line (Ai75) https://www.jax.org/strain/025106, except that I want it to be constituvely expressing the fluorescent protein.
I am specifically interested in cortical neurons, therefore if expression is specific to neurons it would be a plus, but I don't mind that much if the nuclear marker is expressed in other cells.
I could also use a viral approach, but the problem would be that I would like to avoid possible toxic effects due to unregulated over expression typically observed with viral transfections (at least in my experience with AAVs in mice cortical neurons)
Alternatively I would also consider using a fluorescent dye such as DAPI, but it should be in the red spectrum, longlasting (a month or so) and non-toxic :D. As I said before, it is for a two-photon microscopy in vivo experiment.
Also I wonder if anyone knows whether there are any studies showing the toxicity of fluorescent proteins such as mRuby2 or tdTomato targeted to the nucleus with nls or H2B?
Thanks!
- Badges
- Science topic
- Similar topics
- Gels