Are my cells contaminated with mycoplasma?

I suspect contamination with mycoplasma and fixed the cells with 4%PFA and stained them with Dapi. This is the image I got, I usually don't get any of those small rounded dapi foci outside the...

06 August 2020 6,412 1 View

Do you think improved varieties and better management practices will be the key to future increases in food production?

Agricultural experts generally agree that future increases in food production will come from three options:1) expanding the land area, 2) increasing the frequency in which the land is cropped, and...

03 July 2020 8,215 4 View

Do you think Golden Rice will alleviate hunger and malnutrition, especially in developing countries?

Golden Rice is a GMO rice plant that was modified to produce beta-carotene (provitamin A) in its grain. It was invented by the German scientists Prof. I. Potrykus (ETH) and Prof. P. Beyer (Univ...

20 June 2020 8,154 54 View

Is sustainable agriculture the same as ecological agriculture?

"Sustainable agriculture" and ecological agriculture" are two very popular terms for both government and non-government workers. Many people use them interchangeably thereby causing a lot of...

01 June 2020 1,624 100 View

What is the explanation for the "no correlation" between soil test and plant tissue test values?

In our recent research on sweetpotato here in Leyte, Philippines, we have observed a no correlation between soil test and plant tissue test values for N, P and K. Marschner (1995) in his classic...

27 April 2020 5,357 18 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

How to estimate the penetration thickness of light in a solid sample?

I have a solid sample and I need to determine the depth of light penetration to produce a pellet and elucidate some optical phenomenons.

27 May 2019 3,067 1 View

What is the reason for the wide price range of Carbon Nanotubes? How to assess the quality of CNTs?

Suppliers of carbon nanotubes (SWCNT, DWCNT, MWCNT) provide very different quotes for their product which, at an industrial level, should be related with their quality: - How to assess the quality...

05 December 2018 5,514 3 View

How can I determine the method from a .raw file?

I have raw files from a ltq-velos orbitrap but I would like to know the running method used, for instance the resolution at ms1 and ms2. (Proteomics/mass-spec)

22 August 2018 6,887 4 View

No title

15 March 2018 4,243 7 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View