Are my cells contaminated with mycoplasma?

I suspect contamination with mycoplasma and fixed the cells with 4%PFA and stained them with Dapi. This is the image I got, I usually don't get any of those small rounded dapi foci outside the...

06 August 2020 6,412 1 View

Do you think improved varieties and better management practices will be the key to future increases in food production?

Agricultural experts generally agree that future increases in food production will come from three options:1) expanding the land area, 2) increasing the frequency in which the land is cropped, and...

03 July 2020 8,215 4 View

Do you think Golden Rice will alleviate hunger and malnutrition, especially in developing countries?

Golden Rice is a GMO rice plant that was modified to produce beta-carotene (provitamin A) in its grain. It was invented by the German scientists Prof. I. Potrykus (ETH) and Prof. P. Beyer (Univ...

20 June 2020 8,154 54 View

Is sustainable agriculture the same as ecological agriculture?

"Sustainable agriculture" and ecological agriculture" are two very popular terms for both government and non-government workers. Many people use them interchangeably thereby causing a lot of...

01 June 2020 1,624 100 View

What is the explanation for the "no correlation" between soil test and plant tissue test values?

In our recent research on sweetpotato here in Leyte, Philippines, we have observed a no correlation between soil test and plant tissue test values for N, P and K. Marschner (1995) in his classic...

27 April 2020 5,357 18 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

How to estimate the penetration thickness of light in a solid sample?

I have a solid sample and I need to determine the depth of light penetration to produce a pellet and elucidate some optical phenomenons.

27 May 2019 3,067 1 View

Where can I find information about contractual practices that stimulate performance in urban transport by bus?

I would like to learn more about the contract design of urban transport by bus that stimulate competition and better performance. Among the elements that I would like to learn more about...

02 April 2019 7,562 1 View

What is the reason for the wide price range of Carbon Nanotubes? How to assess the quality of CNTs?

Suppliers of carbon nanotubes (SWCNT, DWCNT, MWCNT) provide very different quotes for their product which, at an industrial level, should be related with their quality: - How to assess the quality...

05 December 2018 5,514 3 View

How can I determine the method from a .raw file?

I have raw files from a ltq-velos orbitrap but I would like to know the running method used, for instance the resolution at ms1 and ms2. (Proteomics/mass-spec)

22 August 2018 6,887 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View