Hi all,

Recently our lab moved to a new lab with a new culture room (and new equipment).

We routinely do transient transfection to express membrane proteins in HEK293T cells. For some reason, however, the transfection yield decreased in the new culture room.

We are using the same cells and reagents as before and the incubator seems to be working fine. We also did mycoplasma test and it was negative,

Has anyone experienced anything similar or has any idea what could be causing this change?

Thank you in advance,

Raviv Dharan