How has Covid-19 Pandemic effected the article reviewing process in scientific journals?

For editors of scientific journals, is it a normal duration to keep authors waiting for more than 3 months for making a decision about whether thier article will be accepted for reviewing after...

24 November 2020 270 23 View

Can you give information about how to calculate the molarity of MEA?

Hi, I am trying to produce CZTS films by sol-gel spin technique. Can you give information about how to calculate the molarity of MEA? Did you calculate the MEA molarity separately for each metal...

11 July 2020 3,415 1 View

Does anyone have experienced hanging drop method?

I've done hanging drop with A549 cells. (10 ul/20.000 cell/drop) At second day I saw a few cells have attacht on lid, behind the sphere colony. At third day they become much more cells that...

15 January 2018 4,930 11 View

How can I change number of my kernel density estimation values without changing 'std'?

Hello eveyone, I have mean value is 60, and standard deviation is 10. I'm generating 50 and 800 samples to see kernel. From kernel density estimation in Matlab, I'm getting high 'std' like 20ish...

25 June 2017 8,038 1 View

What are the best conditions for sonication for ChIP assay?

I'm going to use abcam's ChIP assay protocol. At the sonication step it just talk about times. (5-10-15 min. etc.) But there's nothing about power or cycle. What do you prefer for 500-600 bp dna...

30 August 2015 2,944 3 View

Are some distributions convergent to the Gaussian distribution?

I have read that "many" distributions converge to Gaussian distributions. The conditions mentioned along with are number of trials should be large enough and mean as well as variance should...

25 November 2014 7,552 2 View

Out-of-Sample Period: Testing vs. Validation sample?

In forecasting applications, there are different strategies for sampling based on the method used. In computer intelligence, scholars need to define a longer out-of-sample period and have both...

17 November 2014 2,925 1 View

Random number generator - can anyone help?

I want to know whether the period of random number generator depends on the programming language or the bits in the processor? If it depends on the processors than how the periods or randomness...

09 August 2014 3,365 8 View

What number of points is required to fit Gaussian Distributions?

A Gaussian distribution has an exponential term and 2 variables. Its log will turn it linear. But I am not able to figure out how many points will suffice to fit a gaussian distribution and why?...

26 March 2014 8,244 8 View

What are the storage conditions for PBS-Azide-TAME buffer?

16 June 2013 2,717 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View