Primer designing from reverse translation of protein sequence

Hi, I am interested to design primers for my protein of interest (3' RACE). I have N-terminal amino acid sequence of protein. When I reverse translate that protein, codon usage table is required...

06 July 2017 3,028 4 View

Hi, I need help to defat samples before protein extraction. Should I treat seed samples with hexane only or fresh samples also need treatment?

Hi, I am going to freeze dry samples prior to protein extraction for transport to some other destination. As seeds contain so much oil these are treated to defat them before protein extraction.I...

03 April 2015 6,476 6 View

nested pcr

hi, i am doing nested pcr these days but my outer primers are not giving bright required band but other non-specific dense bands. I am using master mix recipie for 25 microliter pcr reaction...

09 October 2010 6,666 2 View

Nested pcr

Hi, I am doing nested pcr. I have completed round of pcr with outer primers now i am going for nested round of primers should i use pcr reaction as such or dilution of pcr reaction with outer...

09 October 2010 2,092 3 View

PCR

Hi everybody, I have prepared stock of primers in 10X TE buffer by mistake and then prepared working stock in distilled water. My primers are not working what should i do now. Thanx

09 October 2010 3,309 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View