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Questions related from Bushra Javaid
Hi, I am interested to design primers for my protein of interest (3' RACE). I have N-terminal amino acid sequence of protein. When I reverse translate that protein, codon usage table is required...
07 July 2017 3,100 4 View
Hi, I am going to freeze dry samples prior to protein extraction for transport to some other destination. As seeds contain so much oil these are treated to defat them before protein extraction.I...
04 April 2015 6,540 6 View
I am preparing methanolic and aqueous extracts of some plants. After evaporating solvent by rotary evaporator small volume e.g 20 ml is left. I want to ask to which extent I should let it...
02 February 2015 9,848 2 View
hi, i am doing nested pcr these days but my outer primers are not giving bright required band but other non-specific dense bands. I am using master mix recipie for 25 microliter pcr reaction...
10 October 2010 6,768 2 View
Hi, I am doing nested pcr. I have completed round of pcr with outer primers now i am going for nested round of primers should i use pcr reaction as such or dilution of pcr reaction with outer...
10 October 2010 2,153 3 View
Hi everybody, I have prepared stock of primers in 10X TE buffer by mistake and then prepared working stock in distilled water. My primers are not working what should i do now. Thanx
10 October 2010 3,395 3 View
