Hello,
I'm going to establish HCC chemo-resistant cell line. Should I treat the cells only with pure compound of anti-neoplastic drugs (Doxorubicin, Cisplatin, Sorafenib)? What if I use clinical version? Does it give same results?
If it is possible I think you should test the pure compound since it will be more easy to control the precise concentration of the drug added in your cell culture and the way it will be added (DMSO, EtOH, aqueous solution). Furthermore you will avoid interference wirh other products present in the pharmaceutical preparation.
If it is possible I think you should test the pure compound since it will be more easy to control the precise concentration of the drug added in your cell culture and the way it will be added (DMSO, EtOH, aqueous solution). Furthermore you will avoid interference wirh other products present in the pharmaceutical preparation.
Hello Hamed
Yes, it is better to use the pure compound at the IC50 concentration (for exemple), and wait the time necessary to obtain a doubling time similar to the parental line before to increase the concentration, and wait again, ...good luck
pure compound for sure. Start with a low dose and gradually enhance the value to create a resistant line.
Remember: your published papers, are a permanent record of your research, are your passport to your community
The sequence of writing the article is not the same as reading it. For most scientific fields, the most efficient way is to regard the manuscript as a wall, building it brick by brick. You start with producing your tables and figures, with captions/legends.
Then describe the Method used, then the Results found and the Discussion around these results.
The Conclusion should flow on from this fairly easily.
Then you write the Introduction, as to where your work fits into the field as a whole.
Finally, the Title, Abstract and Keywords.
This process of building the article is easier, more logical and more effective that trying to write an article in the sequence it is read.
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