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More Maqsood Bhutto's questions See All

Is the latest ABS journal ranking available on internet? How to get it?

Need latest ABS journal ranking list. Please assist.

17 November 2020 5,125 3 View

Any Book or Article on Pleistocene Planktonic Foraminifera and Calcareous Nannoplankton?

Dear all, I am working on the biostratigraphy of Pleistocene sediments from the high latitude region in south-east Indian ocean sediments. Kindly suggest me any book or articles in which the...

09 September 2020 1,544 9 View

What is the difference between SSCI and SCIE?

Please Explain the difference between the Social Science Citation Index and Science Citation Index Expanded?

12 May 2020 6,371 5 View

Can anyone suggest me some new variables that affect consumer attitude, subject norms, percived behavior control in the context of green products?

I want to extend theory of planned behavior, kindly recommend me some new variables that has been not investigated before. Check the attached model, want to extend model mentioned in picture.

09 December 2019 3,262 5 View

Which Universities of USA offer Post Doc in Islamic Studies?

I am interested in doing Post Doctorate on religious tolerance in any university of USA through Fulbright scholarship. Can someone please suggest names of three universities offering post doctoral...

07 October 2019 890 2 View

What leadership style fosters innovation in top innovative universities ?

I have an idea to work on what leadership style (Transformational, Transactional, Autocratic) fosters innovation in top innovative universities ? What is your feedback, is it good enough for one...

14 March 2019 4,680 16 View

I want to know what factors influence organizational Innovation in academic institutions???

What factors can facilitate and bother innovation in academic institutes and how leaders in universities can sustain and foster innovation ?

10 March 2019 1,477 32 View

How can I do LCF Analysis using "direct Cycle" in Abaqus/cae?

hei all, A sheet (Stainless Steel sheet 10mm thick) bending with Die and Punch (V-Shape), and I want to simulate LCF analysis using "Direct cycle" approach and calculate life cycle of die and...

28 September 2018 6,118 3 View

Hi everyone. I want to use aCSF media for stem cells culture. just aCSF is enough to be used for culture or this need any additives?

Just aCSF media will work or need some additives? Regards

04 December 2017 1,002 3 View

Why am I getting polymer solution drops instead of Fibers?

I am working on electrospinning of Carboxymethyl Chitosan. I always get drops of polymer solution on Aluminium Foil instead of fibers. I think this is because of High surface tension. Is this true ?

25 September 2014 5,895 13 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

How can AI be used for enabling Design for Sustainability?

Design for Sustainability (DfS) is a wicked and complex problem. It needs the designer to combine specialized knowledge from several disciplines with systemic thinking. The designer also needs to...

05 February 2021 2,998 9 View

Kulvir Singh

It is not clear to whom or for which project you are asking for.

Please elaborate the question by mentioning some more details.

Emmanuel V Murray

Dear Maqsood,

You are working on a very important & current topic which is of interest to me. If I can be of any help, I am available.

Best Regards

Emmanuel

Ataul Karim Patwary

very interesting topic. Ready for collaboration..