Our lab instrument for qPCR is BIo-RAD CFX connect with CFX maestro program installed in it. Previously our system was set on SYBR. Recently, I started working on TaqMAn
1: TaqMan FAst advanced master mix (Rox inert dye)
2: TaqMan gene expression assays (predesigned probes/primers)(FAM-Report dye)(Tm=60)
My question is
1: How can I set the qPCR system for TaqMan while keeping the settings for SYBR as well?
2: How to analyse the data of gene expression using TaqMan. Is it the same as sybr i.e., delta delta Ct
Manuel Alonso Garrido
Hi Zaib,
Did you manage to solve the question? I want to start using taqman on CFX Opus 384 wells and can find information about how to setting up taq-man probes. Thank you!
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