Can I use the calibration equation which was generated using the salt form of a compound, to detect the base form of compound ?

20 February 2016 5,495 1 View

PCR with single primer

Hi, Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong... And, supposedly the...

11 March 2009 7,200 6 View

assays and data interpretation

Hi, there was recently a question about real time results and how to interpret them. I would recommend the paper: Quantification of mRNA using real-time RT-PCR, from Nolan et al., Nature...

05 February 2009 9,316 0 View

T4 RNA Ligases

Hi, I have recently been a bit confused by the T4 RNA ligases... I know they are being used for 5' and 3' RACE, and also for RNA end labeling... Does anyone know if I can also use them to tag...

03 February 2009 8,263 1 View

Polyadenylation+RT-PCR

Hi, I have been trying to reverse transcribe total bacterial RNA by polyadenylation and oligod(T) primers... I used the polyA polymerase kit from Epicentre, cleaned up the reaction on spin...

30 November 2008 8,518 4 View

BSSE and counterpoise correction in solvent

I am confused about how to run a counterpoise-corrected calculation of BSSE in a solvent. ( Actually, I mean iefpcm,solvent=water in the Gaussian 09) Or is the solvent effect important for BSSE?

01 January 1970 2,844 3 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View

What is the best way to minimize/prevent the effects of rigor mortis in excised mouse muscle?

I'm planning to excise mouse muscle to use for ex vivo mechanical testing. What is the best way to prevent the stiffening effects of rigor mortis from confounding results if I cannot isolate the...

30 January 2021 1,641 2 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

The hospitals reported rise in ICU admissions of young COVID patients - Is the new strain of COVID to be blamed?

05 January 2021 3,141 12 View

Any good methods for double-stained markers quantifications from tissue sample using computer programs?

How can we best quantify double-stained markers when comparing diseased and control? For example, if we want to stain one marker of smooth muscle cells and compare the expression in diseased and...

21 December 2020 7,389 1 View

Why there are multiple spikes around 50Hz (Power Interference) in frequency domain (fft)?

I am working on a project related to sEMG classification. In order to process the signal, I am trying to filter the signal, especially power interference. The signal is acquired from Delsys...

07 December 2020 9,745 10 View

Vacuum freeze drying protocol in human skeletal muscle tissue methods, anyone?

I will be freeze-drying small skeletal muscle samples (~60mg) per sample using a Virtis SP Scientific Benchtop Pro Freeze Dryer. I've had difficulty findings a reference protocol to determine the...

21 November 2020 1,423 1 View

Does anyone know of a good PAX7 antibody for IF - not from the DSHB?

Hi, Although I would dearly love to obtain a PAX7 Ab from DSHB, it is not on our university ordering system (and it takes too long to add it). So I wondered if anyone had good experience of a PAX7...

03 November 2020 7,003 2 View