Is it suitable to use a density-based solver to solve supercritical CO2 laminar flow?

We have been numerically investigating the thermal-fluid characteristic of sCO2 laminar flowing in a 0.5 mm diameter circular microtube in upward direction. Depending on sharp density change along...

29 June 2020 5,199 2 View

What is your pump manufacturer recommendations, ensuring low flow rate and high operating pressure?

We have recently designed an experimental setup to investigate the detailed thermofluidic mechanism of near-critical CO2. We need to pump, ensuring low flow rate, high operating pressure and also...

22 July 2018 3,359 3 View

Can I use the calibration equation which was generated using the salt form of a compound, to detect the base form of compound ?

20 February 2016 5,495 1 View

What is the precautions to avoid the negative effects of hydrogen sulphide on heat exchanger copper fin?

23 August 2015 2,445 3 View

How can I calculate enthalpy uncertainty value which is necessary for laboratory refrigerant side capacity?

27 April 2015 8,148 8 View

Does anyone know NIST Evap-Cond V.3.0 circuity design program?

13 November 2014 4,994 0 View

PCR with single primer

Hi, Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong... And, supposedly the...

11 March 2009 7,200 6 View

assays and data interpretation

Hi, there was recently a question about real time results and how to interpret them. I would recommend the paper: Quantification of mRNA using real-time RT-PCR, from Nolan et al., Nature...

05 February 2009 9,316 0 View

T4 RNA Ligases

Hi, I have recently been a bit confused by the T4 RNA ligases... I know they are being used for 5' and 3' RACE, and also for RNA end labeling... Does anyone know if I can also use them to tag...

03 February 2009 8,263 1 View

Polyadenylation+RT-PCR

Hi, I have been trying to reverse transcribe total bacterial RNA by polyadenylation and oligod(T) primers... I used the polyA polymerase kit from Epicentre, cleaned up the reaction on spin...

30 November 2008 8,518 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View