Tnf alpha or Tnf beta, which should be assayed for Th1 cytokines?

More Chinwe Ewenighi's questions See All

After an error questionairee is discarded what happens next?

If a sample size is 300 and 5 Questionnaires are discarded due to error, are the 5 discarded questionairee not meant to be replaced? Just curious

16 April 2024 7,472 3 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,565 5 View

Is any one experienced in implementation research (IR)?

I am working on vaccines supply chain and I need assistance from someone who is experienced in IR. I am seeking for the best strategies to improve the supply chain and how to implement the...

16 February 2017 5,143 3 View

Do immunoglobulin A, G, M have any relevance in the "prevention from mother to child transmission of HIV" ?

Do immunoglobulin A, G, M have any relevance/importance/role in the "prevention from mother to child transmission of HIV"

06 July 2016 8,395 2 View

Can the immune system of HIV+ mothers determine if the child becomes HIV+ or not?

please i want an article that proved that the immune strength of HIV+ mother determines if the infant becomes HIV infected or not

05 July 2016 2,114 5 View

Literature elucidating the role of total plasma IgG, IgA and IgM in HIV infected HAART treated and HAART naive pregnant mothers towards PMTCT?

I have found out that there is scarcity of information concerning the role of total plasma IgG, IgA and IgM in HIV infected HAART treated and HAART naive pregnant mothers towards the prevention of...

21 March 2016 9,203 1 View

Can anyone assist with a question on Shiga toxin producing E.coli?

I am working on Shiga toxin producing E.coli (STEC). I have presumptive isolates and i want to determine their sero groups, that is O157 and non O157 as well as their virulent genes, stx, eae,...

31 August 2014 1,074 9 View

Under what conditions do substances that increase the activities of antioxidant enzyme induce stress?

Substances that suppress antioxidant enzyme (SOD, CAT,etc) activity induce oxidative stress. Under what conditions do substances that increase the activities of these enzyme induce stress? A...

27 January 2014 4,149 3 View

What is the mechanism of induction of dyslipidemia in rat model by Triton WR1339?

I want to study the hypolipemic potentials of a medicinal plant.

22 September 2013 1,845 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Tapan kumar Mukherjee

Use TNF-beta for TH1 cytokines

Manoranjan Sharma

TNF-beta will be best, for better confirmation IL-2 along with TNF-beta will further establish Th1 dominance.

Jang-June Park

Both TNF alpha and TNF beta are Th1 effector function. The differences in the biological activity between TNF alpha and TNF beta are based on different abilities for binding to the target cells.

Johnny Atman

Inteferon gamma?