What technique to use to look for differences in two independent samples of count data?

Hi everyone, I have a sample of prices of products across different competitors. I am trying to compare the pricing across each one with the goal of looking, which company is significantly...

15 September 2018 7,980 3 View

CFU-F assay?

I like to perform a CFU-F assay for MSC. If I understood well this takes some 2 weeks of culturing before colonies are formed. During this culture do you replace the medium or can you leave the...

04 March 2018 5,694 3 View

Can any one recommend me a program/software or a tool to draw the optical setups, in the interest of using them for a technical paper ?

Hi, I am searching for a tool which would help me to draw the optical setups and schematics better than Microsoft powerpoint. I am attaching the setup which I have drawn using powerpoint; however,...

21 January 2016 4,602 11 View

Can anyone help me with assembly annotation and submitting chloroplast whole genome sequencing project?

Micro algae chloroplast DNA.

27 December 2013 6,692 9 View

Does anyone have experience with detection of fungus Trichoderma harzianum through qPCR?

I need to detect the fungus Trichoderma harzianum with a qPCR. I know the strain (Trichoderma harzianum Rifai T22), but when I search the NCBI website for primer development I get stuck. When I...

24 October 2013 8,844 5 View

Why doesn't a qPCR reaction need to have an Elongation step?

Why doesn't a qPCR use an elongation step while a normal PCR reaction does next to its hybridization step? Is it because the fragments are smaller in a qPCR? Thank you for your time, Bas

15 July 2013 1,943 3 View

Straight line curve in q-PCR.

Attached is my file from a Biorad CFX96 Real Time system. The red lines are standard curves, the blue curves are monsters with different concentrations and the green curve is a negative...

26 May 2013 3,309 18 View

Why and when do synapses fail after ischemia?

I am building a numerical model of the neural activity after a stroke. My observation from the literature is that synapses fail typically ten seconds after interruption of blood flow. I found...

06 July 2012 1,506 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View