Is there a protein extraction protocol from the intestinal content of fish?

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Mohammad Tariq Ali Khan

There is no specific protocol but I can suggest you something if you want to try.

Collect everything in 15 mL falcon.

Spin at maximum 2000rpm for 5 minutes.

Take supernatant in separate 15 ml tube and reamining pellet.

Add 1-2mL PBS to pellet and centrifuge (2000rpm for 2 min) it along with supernatant tube.

Remove PBS and add 100-200uL protien lysis buffer depening on pellet size.

To the supenatant tube, dsicard liquid, add PBS 1-2mL, wash it by spinning at 2000rpm for 2 min.

Add 100uL protien lysis buffer.

Note: Do all steps in ice bucket or on ice. To avoid degradation of proetin.

Find attached protocols.|

Taking pellet and supernatant both in separate tube will help you next time to find which part has protein.

Steingrimur Stefansson

Hi Alexandros, I have purified trypsin, chymotrypsin and alkaline phosphatase from cod guts on a semi-industrial scale using basic methods and not kits.

What are you after?