How do I interpret fit statistics SEM and Modindices output?

Hi, I would need some help in understanding the SEM output. I run a latent change score analysis to test the change score of a variable of interest at two-time points (repeated measure) PLD1 and...

05 January 2021 3,367 2 View

Lipofectamine 3000 in 10 cm dish?

Dear all, I'm trying to transfect HeLa cells with lipofectamine 3000 in a 10 cm dish. Do someone of you have a protocol to share? I still examined the table for scaling up or down lipo3000 but it...

13 October 2020 2,926 3 View

Which is the best system to keep endocrinology samples frozen in the field, without electricity?

Hi everyone, I am looking for suggestions to store biological samples in the field for endocrinology analyses, where I will not have electricity. One of the options we are evaluating is a solar...

01 September 2020 3,766 3 View

How to combine beam and shell elements in ANSYS APDL?

Hi everyone! I have a doubt and I hope there is someone who can give me an advice. I am modeling a MEMS gyroscope for my Master thesis and I am starting with a very basic structural design. My...

20 August 2020 9,524 5 View

How to edit density and heat capacity in Aspen Plus ?

Recently I'm trying to edit a pure component mass density and heat capacity of an existing component NaNO3 but failed. Could anyone help me on this? Thanks a lot.

17 July 2020 8,800 8 View

Do you have suggestions about CROSS CULTURAL ADAPTATION of questionnaires?

Hi! I am currently translating and adaptating a questionnarire about attitudes towards inclusion, and I would like to have suggestions about specific literature in translation and cross-cultural...

23 March 2020 2,630 3 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene in a common cell line?

01 March 2020 3,206 2 View

Does CXCL8 or other chemokines induce in vitro cell proliferation?

hi everybody, i have some troubles with chemokines and cell proliferation. Different papers evidence that CXCL8 (but also CXCL12, CCL2 and other chemokines) are able to promote cancer cell...

03 February 2020 214 4 View

Anyone could suggest how to analyze calcium imaging using imagej?

Hi, we would like to analyze time sequences images of calcium imaging assay performed with Fluo4-AM in imagej. Is there any plugin that we could install to do this? Do you have any kind of...

27 January 2020 2,665 1 View

Chloroform and isopropanol RNA extractions?

I have to perform RNA extraction with TRIzol LS and I just realized that we have chloroform stabilized with ethanol. Is that ok? And should chloroform and isopropanol be "molecular biology...

30 September 2019 4,647 5 View

How should I calculate sample size when my research is about normal population? What is difference between sampling frame and sample size?

I am asking this question on behalf of my colleague who has to do thyroid's elasticity scan of normal population. This data will act as reference value in order to compare with diseased one. So,...

23 July 2020 8,303 2 View

Do you have any idea about the clinical significance of diffuse low density of thyroid gland ?

i noticed that the thyroid gland of some patients is quite low, similar to that of muscles. and the CT scan was described for various reasons, even including regular health checks. i tried pubmed,...

29 June 2020 3,238 6 View

Can anyone please name a few cheaply available monomers that can be used along with TPO photoinitiator?

Please do suggest cheaply available monomers that can be used along with TPO photoinitiator and also the appropriate wavelength of light needed to polymerise the particular monomer.

20 April 2020 4,832 3 View

How can we stabilize purified humanThyroid Stimulating Hormone (hTSH) in buffer, without the use of albumin or other large molecules?

We use purified hTSH (in PBS), but have issue with stability of the sample. Since the purified hormone is highly unstable the activity reduces drastically at room temperature. We had added BSA to...

06 July 2019 8,619 4 View

How can I improve Mask RCNN performance for nodule detection in Ultrasound thyroid images?

I am trying to use faster RCNN (in MATLAB) for nodule bounding box detection in a small thyroid Ultrasound dataset (only 233 images). Some nodules are big and some are very small. My question is...

05 June 2019 7,322 1 View

Affordable source of Thyrotropin / bTSH?

I've been trying and failing to obtain cell-culture grade crude TSH (probably bovine) for propagation of laboratory thyroid cell lines (ie. FRTL5) - Merck / Sigma had a suitable product in their...

04 May 2019 6,718 1 View

ELISA kit to assay TPO activity or T3 and T4 concentrations in cell culture supernatants?

Hi everybody, could anyone suggest me a commercial ELISA kit which can be used to assay TPO activity or T3 and T4 concentrations in cell culture supernatants? Francesca

04 May 2019 9,779 3 View

Affordable source of Thyrotropin / bTSH?

I've been trying and failing to obtain cell-culture grade crude TSH (probably bovine) for propagation of laboratory thyroid cell lines (ie. FRTL5) - Merck / Sigma had a suitable product in their...

01 May 2019 1,335 1 View

Could you recommend some kits to measure mice T3/T4/TSH(thyroid hormnes)?

I wonder if there're some Elisa kits which have been identified to test mice T3/T4/TSH, I've searched R&D, and there're only T4 Kit.Or could I use the RIA machine used in clinical testing...

19 March 2019 598 1 View

What are the best supplements for thyroid gland and why?

Ive been researching this topic since a while and I would love to expand my knowledge in this area, Ive come across some studies claiming that selenium is effective in treating thyroid...

01 February 2019 4,003 18 View