can anyone suggest me how to remove lacZ promoter from puc18 inorder to clone any other promoter over there and to study the expression of lacZ under new promoter influence??
I want to know about the calculation of osmolality of phosphate buffer pH 7.0. Similarly, Osmolality of 450 mM sucrose and lactose. As they are non-dissociated compounds, should they refer to 450...
04 May 2016 2,479 2 View
I run a sample (containing lots of compounds) on hplc column to see my required product. Now I want to extract my pure compound peak from Column. I want to know the methodology. If some one has...
02 March 2015 7,149 7 View
I want to summarize the data of whole transcriptome sequencing of bacteria according to their classes like oxidoreductases, membrane proteins, genes linked to fatty acid synthesis etc. Is there...
07 August 2014 5,741 4 View
I need 5 keto fructose as standard in HPLC. I tried different companies but not successful to buy it. Can anyone send me some link from where I can buy it easily.
05 June 2014 3,113 2 View
I have PEG in bacterial culture as osmoticum. I find the difference of half in term of O.D of stress versus control culture. On dry mass, there was difference of 10-15mg/100ml culture. Substrate...
04 May 2014 8,267 5 View
I want to calculate osmolarity of 10%PEG in media (yeast extract 0,6%, Mannitol 2%). I want to make a hypotonic buffer to get compatible solutes from bacterial pellet by milking. I need mosm/l of...
04 May 2014 5,972 3 View
I have a plasmid with a strong promoter and a gene with a signal peptide. It works perfectly so far for expression. Now I want to clone one more gene in the same plasmid under the same promoter...
04 May 2014 7,984 3 View
I am working on osmotic stress in a member of family alpha-proteobacteriaceae. My organism keeps doubling time constant but there is a visible effect on end O.D. I used 10% PEG as osmotic stress...
02 March 2014 4,662 2 View
What is the benefit of index files and .bam files? How can we get the information from contig reads. What does coverage stands for and how is ti calculated?
10 November 2013 6,923 1 View
i want to dock copper on my protein, which has metal binding site. can you please guide me step wise, how to perform this??
04 May 2011 6,131 15 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View