13 Questions 16 Answers 0 Followers
Questions related from Nageena Zahid
I want to know about the calculation of osmolality of phosphate buffer pH 7.0. Similarly, Osmolality of 450 mM sucrose and lactose. As they are non-dissociated compounds, should they refer to 450...
05 May 2016 2,444 2 View
I run a sample (containing lots of compounds) on hplc column to see my required product. Now I want to extract my pure compound peak from Column. I want to know the methodology. If some one has...
03 March 2015 7,119 7 View
I want to summarize the data of whole transcriptome sequencing of bacteria according to their classes like oxidoreductases, membrane proteins, genes linked to fatty acid synthesis etc. Is there...
08 August 2014 5,715 4 View
I need 5 keto fructose as standard in HPLC. I tried different companies but not successful to buy it. Can anyone send me some link from where I can buy it easily.
06 June 2014 3,084 2 View
I want to calculate osmolarity of 10%PEG in media (yeast extract 0,6%, Mannitol 2%). I want to make a hypotonic buffer to get compatible solutes from bacterial pellet by milking. I need mosm/l of...
05 May 2014 5,946 3 View
I have PEG in bacterial culture as osmoticum. I find the difference of half in term of O.D of stress versus control culture. On dry mass, there was difference of 10-15mg/100ml culture. Substrate...
05 May 2014 8,238 5 View
I have a plasmid with a strong promoter and a gene with a signal peptide. It works perfectly so far for expression. Now I want to clone one more gene in the same plasmid under the same promoter...
05 May 2014 7,958 3 View
I am working on osmotic stress in a member of family alpha-proteobacteriaceae. My organism keeps doubling time constant but there is a visible effect on end O.D. I used 10% PEG as osmotic stress...
03 March 2014 4,639 2 View
What is the benefit of index files and .bam files? How can we get the information from contig reads. What does coverage stands for and how is ti calculated?
11 November 2013 6,890 1 View
i want to dock copper on my protein, which has metal binding site. can you please guide me step wise, how to perform this??
05 May 2011 6,105 15 View
i want to know whether we can use dh5 apha as expression host or not. most of the people use BL21 strain as host. secondly can anyone tell me about BL21 and JM strains are mutant for lacZ genes or...
10 October 2010 3,908 0 View
can someone tell me anysuitable expression host except DH5alpha having lacZ mutation and does allow any type of recombination.??
10 October 2010 4,348 2 View
can anyone suggest me how to remove lacZ promoter from puc18 inorder to clone any other promoter over there and to study the expression of lacZ under new promoter influence??
07 July 2010 599 0 View
