Nageena Zahid

13 Questions 16 Answers 0 Followers

Questions related from Nageena Zahid

Osmolality of buffers and organic solutes?

I want to know about the calculation of osmolality of phosphate buffer pH 7.0. Similarly, Osmolality of 450 mM sucrose and lactose. As they are non-dissociated compounds, should they refer to 450...

05 May 2016 2,437 2 View

Can anyone suggest how I can get the specific peak resolved by HPLC from mixed sample in pure form?

I run a sample (containing lots of compounds) on hplc column to see my required product. Now I want to extract my pure compound peak from Column. I want to know the methodology. If some one has...

03 March 2015 7,112 7 View

How to categories the data of next generation sequencing for bacteria with respect to their functions or common classes?

I want to summarize the data of whole transcriptome sequencing of bacteria according to their classes like oxidoreductases, membrane proteins, genes linked to fatty acid synthesis etc. Is there...

08 August 2014 5,710 4 View

Can someone suggest a source where to get 5 keto fructose as a standard in HPLC from?

I need 5 keto fructose as standard in HPLC. I tried different companies but not successful to buy it. Can anyone send me some link from where I can buy it easily.

06 June 2014 3,078 2 View

How can we calculate mosm/l for 10% polyethylene glycol(6000) in media?

I want to calculate osmolarity of 10%PEG in media (yeast extract 0,6%, Mannitol 2%). I want to make a hypotonic buffer to get compatible solutes from bacterial pellet by milking. I need mosm/l of...

05 May 2014 5,941 3 View

What could be the possible reason to avoid osmotic stress by bacteria?

I have PEG in bacterial culture as osmoticum. I find the difference of half in term of O.D of stress versus control culture. On dry mass, there was difference of 10-15mg/100ml culture. Substrate...

05 May 2014 8,233 5 View

Does anyone knows about expressing two proteins in bacterial periplasm under one promoter and one signal peptide in one vector?

I have a plasmid with a strong promoter and a gene with a signal peptide. It works perfectly so far for expression. Now I want to clone one more gene in the same plasmid under the same promoter...

05 May 2014 7,952 3 View

Can anyone help with osmotic stress and bacterial growth behaviour?

I am working on osmotic stress in a member of family alpha-proteobacteriaceae. My organism keeps doubling time constant but there is a visible effect on end O.D. I used 10% PEG as osmotic stress...

03 March 2014 4,636 2 View

How do I analyse next-generation sequence data using Artemis?

What is the benefit of index files and .bam files? How can we get the information from contig reads. What does coverage stands for and how is ti calculated?

11 November 2013 6,886 1 View

protein ligand docking

i want to dock copper on my protein, which has metal binding site. can you please guide me step wise, how to perform this??

05 May 2011 6,100 15 View

dh5 alpha as expression host

i want to know whether we can use dh5 apha as expression host or not. most of the people use BL21 strain as host. secondly can anyone tell me about BL21 and JM strains are mutant for lacZ genes or...

10 October 2010 3,901 0 View

expression host

can someone tell me anysuitable expression host except DH5alpha having lacZ mutation and does allow any type of recombination.??

10 October 2010 4,337 2 View

this research group helps a lot to new scientists

can anyone suggest me how to remove lacZ promoter from puc18 inorder to clone any other promoter over there and to study the expression of lacZ under new promoter influence??

07 July 2010 594 0 View