Can someone suggest a source where to get 5 keto fructose as a standard in HPLC from?

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Osmolality of buffers and organic solutes?

I want to know about the calculation of osmolality of phosphate buffer pH 7.0. Similarly, Osmolality of 450 mM sucrose and lactose. As they are non-dissociated compounds, should they refer to 450...

04 May 2016 2,479 2 View

Can anyone suggest how I can get the specific peak resolved by HPLC from mixed sample in pure form?

I run a sample (containing lots of compounds) on hplc column to see my required product. Now I want to extract my pure compound peak from Column. I want to know the methodology. If some one has...

02 March 2015 7,149 7 View

How to categories the data of next generation sequencing for bacteria with respect to their functions or common classes?

I want to summarize the data of whole transcriptome sequencing of bacteria according to their classes like oxidoreductases, membrane proteins, genes linked to fatty acid synthesis etc. Is there...

07 August 2014 5,741 4 View

What could be the possible reason to avoid osmotic stress by bacteria?

I have PEG in bacterial culture as osmoticum. I find the difference of half in term of O.D of stress versus control culture. On dry mass, there was difference of 10-15mg/100ml culture. Substrate...

04 May 2014 8,267 5 View

How can we calculate mosm/l for 10% polyethylene glycol(6000) in media?

I want to calculate osmolarity of 10%PEG in media (yeast extract 0,6%, Mannitol 2%). I want to make a hypotonic buffer to get compatible solutes from bacterial pellet by milking. I need mosm/l of...

04 May 2014 5,972 3 View

Does anyone knows about expressing two proteins in bacterial periplasm under one promoter and one signal peptide in one vector?

I have a plasmid with a strong promoter and a gene with a signal peptide. It works perfectly so far for expression. Now I want to clone one more gene in the same plasmid under the same promoter...

04 May 2014 7,984 3 View

Can anyone help with osmotic stress and bacterial growth behaviour?

I am working on osmotic stress in a member of family alpha-proteobacteriaceae. My organism keeps doubling time constant but there is a visible effect on end O.D. I used 10% PEG as osmotic stress...

02 March 2014 4,662 2 View

How do I analyse next-generation sequence data using Artemis?

What is the benefit of index files and .bam files? How can we get the information from contig reads. What does coverage stands for and how is ti calculated?

10 November 2013 6,923 1 View

protein ligand docking

i want to dock copper on my protein, which has metal binding site. can you please guide me step wise, how to perform this??

04 May 2011 6,131 15 View

expression host

can someone tell me anysuitable expression host except DH5alpha having lacZ mutation and does allow any type of recombination.??

09 October 2010 4,380 2 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View