Osmolality of buffers and organic solutes?

I want to know about the calculation of osmolality of phosphate buffer pH 7.0. Similarly, Osmolality of 450 mM sucrose and lactose. As they are non-dissociated compounds, should they refer to 450...

04 May 2016 2,388 2 View

Can anyone suggest how I can get the specific peak resolved by HPLC from mixed sample in pure form?

02 March 2015 7,075 7 View

How to categories the data of next generation sequencing for bacteria with respect to their functions or common classes?

I want to summarize the data of whole transcriptome sequencing of bacteria according to their classes like oxidoreductases, membrane proteins, genes linked to fatty acid synthesis etc. Is there...

07 August 2014 5,681 4 View

What could be the possible reason to avoid osmotic stress by bacteria?

I have PEG in bacterial culture as osmoticum. I find the difference of half in term of O.D of stress versus control culture. On dry mass, there was difference of 10-15mg/100ml culture. Substrate...

04 May 2014 8,199 5 View

How can we calculate mosm/l for 10% polyethylene glycol(6000) in media?

I want to calculate osmolarity of 10%PEG in media (yeast extract 0,6%, Mannitol 2%). I want to make a hypotonic buffer to get compatible solutes from bacterial pellet by milking. I need mosm/l of...

04 May 2014 5,914 3 View

Does anyone knows about expressing two proteins in bacterial periplasm under one promoter and one signal peptide in one vector?

I have a plasmid with a strong promoter and a gene with a signal peptide. It works perfectly so far for expression. Now I want to clone one more gene in the same plasmid under the same promoter...

04 May 2014 7,922 3 View

Can anyone help with osmotic stress and bacterial growth behaviour?

I am working on osmotic stress in a member of family alpha-proteobacteriaceae. My organism keeps doubling time constant but there is a visible effect on end O.D. I used 10% PEG as osmotic stress...

02 March 2014 4,598 2 View

How do I analyse next-generation sequence data using Artemis?

What is the benefit of index files and .bam files? How can we get the information from contig reads. What does coverage stands for and how is ti calculated?

10 November 2013 6,861 1 View

protein ligand docking

i want to dock copper on my protein, which has metal binding site. can you please guide me step wise, how to perform this??

04 May 2011 6,070 15 View

expression host

can someone tell me anysuitable expression host except DH5alpha having lacZ mutation and does allow any type of recombination.??

09 October 2010 4,311 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View