Osmolality of buffers and organic solutes?

I want to know about the calculation of osmolality of phosphate buffer pH 7.0. Similarly, Osmolality of 450 mM sucrose and lactose. As they are non-dissociated compounds, should they refer to 450...

04 May 2016 2,388 2 View

Can anyone suggest how I can get the specific peak resolved by HPLC from mixed sample in pure form?

02 March 2015 7,075 7 View

How to categories the data of next generation sequencing for bacteria with respect to their functions or common classes?

I want to summarize the data of whole transcriptome sequencing of bacteria according to their classes like oxidoreductases, membrane proteins, genes linked to fatty acid synthesis etc. Is there...

07 August 2014 5,681 4 View

Can someone suggest a source where to get 5 keto fructose as a standard in HPLC from?

I need 5 keto fructose as standard in HPLC. I tried different companies but not successful to buy it. Can anyone send me some link from where I can buy it easily.

05 June 2014 3,042 2 View

What could be the possible reason to avoid osmotic stress by bacteria?

I have PEG in bacterial culture as osmoticum. I find the difference of half in term of O.D of stress versus control culture. On dry mass, there was difference of 10-15mg/100ml culture. Substrate...

04 May 2014 8,199 5 View

Does anyone knows about expressing two proteins in bacterial periplasm under one promoter and one signal peptide in one vector?

I have a plasmid with a strong promoter and a gene with a signal peptide. It works perfectly so far for expression. Now I want to clone one more gene in the same plasmid under the same promoter...

04 May 2014 7,922 3 View

Can anyone help with osmotic stress and bacterial growth behaviour?

I am working on osmotic stress in a member of family alpha-proteobacteriaceae. My organism keeps doubling time constant but there is a visible effect on end O.D. I used 10% PEG as osmotic stress...

02 March 2014 4,598 2 View

How do I analyse next-generation sequence data using Artemis?

What is the benefit of index files and .bam files? How can we get the information from contig reads. What does coverage stands for and how is ti calculated?

10 November 2013 6,861 1 View

protein ligand docking

i want to dock copper on my protein, which has metal binding site. can you please guide me step wise, how to perform this??

04 May 2011 6,070 15 View

expression host

can someone tell me anysuitable expression host except DH5alpha having lacZ mutation and does allow any type of recombination.??

09 October 2010 4,311 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View