I'm trying to find a good protocol to co-stain Alzheimer's disease tissue with thioflavin S and 4G8 antibody with IHQ. I haven't found any protocol to perform these two stainings together. I do not want IF, I need to use HRP-secondary antibody. I'd really appreciate it if anyone has suggestions.
Thanks!
Dear Sofia Sepulveda , interesting Q you are asking ...unfortunately - IMHO - lacking most of the 'important' parameters...as there are, for example:
- tissue (animal, human, guessing brain)
- pretreatment (PFPE, cryofix-cryosections)
- Staining proper [which immunostaining (HRP pre/post) /which dye *) see bottom ]
If you can't come back here with some 'elucidation' I'd rather point you to use the Full text Search function in RG-Archives for e.g. >Publications< and / or >Questions< (you find this function in the >middle< top menue of this particular (Q/A, or any other RG-)web-page displaying the text:
>> Search for research, journals, people, etc....
Helo Sofia,
the problem is that thioflavine S is a fluorescence dye. If you prefer to work with brightfield images you have to switch either to congo red which stains the amyloid plaques in a similar way as thioflavine or to an antibody against Amyloid beta. I would recommand a double labeling immunohistochemistry especially when you like to qunatify your results.
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