Separation of alkaloids on thin layer chromatography:

Thin layer chromatography was done according to the method described by Baerheim and Verpoorte (2009) as follows:

Plant material (3 g) was mixed with 10% ammonia solution and extracted with 25 mL ethanol and was kept for 24 h in order to get alkaloid extract. The extract was evaporated to yield a sticky residue. Using a capillary tube, 3 drops of plant extract were placed on each spot at the starting point of TLC plate so that they line up with the notches etched for each plant species. Enough volume of the developing liquid which was a mixture of ethylacetate, methanol, and water (100:13.5:10) was poured in a jar so as to reach 1 cm deep at the bottom. A piece of filter paper was placed into the jar to saturate the atmosphere with the solvent. The plate was then dipped carefully in the developing solvent and the chromatogram was allowed to develop. Plates were removed from the chamber and the highest solvent level on them was traced with a pencil. Also the spots were traced while held under a UV lamp. The spots were outlined with a pencil. The Rf values were then calculated for each spot.

Before running TLC, it better and easer to detect using chemical methods:

Test for flavonoids: Sawant, R. and Godghate, A. (2013) ,International Journal of Science, Environment and Technology, 2(4),pp.634-641.

Test for Alkaloids: Saxena, J. and Sahu, R. (2012), IRJP., 3(8), pp. 203-204.

If gave positive tests, either separate by Flash Chromatography then identify by spectroscopic methods OR match with standers on TLC.

Check https://academicjournals.org/article/article1427281373_Hassan%20et%20al.pdf

Also check https://www.imedpub.com/articles/phytochemical-screening-and-thin-layer-chromatographic-studies-of-guierasenegalensis-gf-gmel-egyptian-mimosa.pdf

i agree with Chinaza Godswill Awuchi follow

https://www.researchgate.net/deref/https%3A%2F%2Fwww.imedpub.com%2Farticles%2Fphytochemical-screening-and-thin-layer-chromatographic-studies-of-guierasenegalensis-gf-gmel-egyptian-mimosa.pdf