We have linked aminobenzoic acid dissolved in PBS 1x with SPDP (succinimidyl 3-(2-pyridyldithio)propionate) dissovled first in a small volume of DMSO (3% of the total reacton volume). We want to isolate the linked reaction product from the reactants by thin layer chromatography.
1. Do we have to perform an extractive workup first or can we apply to reaction mixture directly on TLC platess?
2. What would be the best solvent choice for elution?
Thank you
Parmjeet Kaur
first you have to do extractive workup with ethyl acetate and water, afterwards collect the ethyl acetate layer, dry it over sodium/magnesium sulfate, evaporated under vacuum. Then you dissolve the reaction mixture in appropriate solvent and subject it over TLC plate.
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