Dear Irene Bissoli ,

I am assuming the cell density was low after thawing. The cells are fine in general but a bit stressed. I guess they will recover (within a passage or two perhaps). If in the worst case you observe iPSC colonies begin to differentiate randomly then you can either thaw a new vial / try to pick up the good iPSC colonies and sub-clulture them.

Best wishes

Shambhabi Chatterjee thank you very much!

Hi Irene,

I suggest, whenever you recover iPSC add rock inhibitor (it reduces the cell death). Though cell number looks fine, but not in proper morphology, looks like differentiated ones are more, wait for 1-2 passages and then see cells. If it's still looks like this, do manual passage these cells (means harvesting the proper colony ) and grows.

Hope it works, Good luck