Has anyone used a premade hybridization buffer (from Roche for example) for zebrafish in situ hybridizations?

Our in situs (alkaline phosphatase based) have stopped working. Probes are fine, but or homemade hybridization buffer seems to be the issue. Using the same supplier of deionized formaminde as...

03 February 2021 8,540 2 View

Does anybody have any resources to understand how polymerization kinetics of random copolymers work? Specifically hydrogels if possible?

I am performing rate of reaction studies on hydrogels with 2 monomers and crosslinker. We can look at absorbance of a single wavelength of light over the course of the reaction through UV-Vis to...

29 October 2019 4,443 2 View

What disease pathologies are you aware of that would benefit from elemental analysis?

Hello, I am currently researching a spectral computed tomography system for medical applications. The system excels in delineating the elemental composition of materials, providing a 3D...

06 March 2019 3,465 3 View

Making antisense probe from topo?

I need to make an antisense probe from a cloned sequence in pcrTOPO4. When I sequenced using the m13R primer, the sequence through a BLAST search come back as Plus/Minus. Just wondering how i...

01 March 2017 3,003 3 View

Will anti DIG-AP (roche) work after one freeze thaw cycle?

My anti DIG antibody-AP (Fab fragments, ROCHE, ) were were put in our freezer upon delivery. The product information states to store at 4 degress, and specifically says not to freeze. Anyone know...

27 September 2016 278 2 View

Any recommendations on which macrophage (P388D1 vs RAW 264.7) to use for studying bacterial killing via the oxidative burst?

I have a mutant bacteria that is more tolerant to H2O2 and I would like to test if it is more persistent in the macrophage model? Will either of these cell lines phagocytose and degrade bacteria,...

23 March 2015 9,088 4 View

Can anyone suggest reasons for the appearance of this non-specific band in PCR?

I am working on amplifying the nirK gene from whole community genomic DNA. However I am getting a second band just below my target (500bp) band. The PCR program I am using is below: 94C-5min |...

03 February 2014 7,182 71 View

Great project! How did you go about warming the students up to the rich picturing process, to get them involved?

How did you go about warming the students up to the rich picturing process, to get them involved? I feel that I have never quite cracked the challenge and disbelief from participants in RP...

01 January 1970 1,738 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View