The yield of my LDHA protein is low and functional. As such, is there any way to increase the recombinant protein yield by manipulating the induction?

13 October 2017 1 373 Report

Regarding IPTG induction

There are many methods to optimize protein expression: time, time vs IPTG concentration, temperature, time of induction - cell induction in stationary phase or for same protein in logarithmic phase is better. You can also change expression strain and, aerobic or anaerobic conditions, using protease-deficient host strains and much more. I hope that the attached publication will be useful for you.

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